Agilent MAC Mode Imaging of Biological Molecules with 7500 AFM - Application Note 5991-3672EN c20141020 [2] free download

File name MAC Mode Imaging of Biological Molecules with 7500 AFM - Application Note 5991-3672EN c20141020 [2].

Keysight Technologies MAC Mode Imaging of Biological Molecules with Keysight 7500 AFM Application Brief Introduction examples of high-resolution MAC mode imaging of biological samples in liquid One of the most appealing features of using Keysight 7500 AFM will atomic force microscopy (AFM) for the be demonstrated. field of biology is that it can operate in aqueous environments, making this Instrumentation technique capable of monitoring or measuring biological molecules under The Keysight 7500 AFM/SPM physiological conditions. In terms of microscope is a high-performance AFM imaging, the complexity of probe instrument that delivers high resolution oscillations and tip/sample force imaging with integrated environmental interactions in solutions is much higher control functions. Keysight's patented than that under ambient conditions. For magnetic AC mode (MAC Mode) is Figure 1. MAC mode imaging of lambda phage instance, ordering of the liquid at a solid offered as a system option. Switching DNA in a buffer solution. interface is a ubiquitous phenomenon imaging modes with the Keysight and such liquid structuring could have a profound effect on the operation of an 7500 AFM/SPM microscope is quick Imaging of DNA in and convenient, a result from the AFM, causing the tip to hop between scanner's interchangeable, easy- a Buffer Solution different stable configurations in the to-load nose cones. Every aspect of ordered region of the fluid. A Keysight the Keysight 7500 AFM's design and Lambda phase DNA is used as the first Technologies, Inc. patented magnetic construction are optimized to reduce example to demonstrate the effective AC (MAC) mode is developed as a better mechanical noise, and deliver industry AFM imaging in liquid. When DNA in a approach to AFM in liquid, in which only leading performance. The compact buffer solution containing Ni++ or Mg++ the cantilever part of an AFM probe is and completely encapsulated scanner is added to a mica substrate, these coated with a thin magnetic coating provides easy cantilever exchange, a divalent cations can form a charge and the introduction of an alternating slot for (optional) preamps for STM bridge between the negatively charged magnetic field will induce a direct tip and CSAFM operation, as well as an phosphate groups on the DNA backbone oscillation. This method eliminates integrated, high-reliability connector to and the negatively charged siloxy groups the spurious resonances seen in fluid interface with the control electronics. on the mica. This